Foggy mornin'. Well, things finally worked. I struggled for a week to figure out why I couldn't get a proper I53D mutant of RNase H. I finally figured out how much phosphate was in my PCR buffer, and quickly desalted. Now, my site-directed mutagenesis worked perfectly!

I started a series of minipreps last night, and got up early today so that I could finish the preps before the sequencing people get here at 11:00 am. Which brings me to my point. Today is the first day this fall that I've been up early enough to see the fog before it rolled back. I caught a shot of the Campanile in the fog with the ol' cell phone. This (from what I hear) is typical Berkeley, early morning.